Kinetics of Dermatophagoides pteronyssinus and Dermatophagoides farinae growth and an analysis of the allergen expression in semi-synthetic culture medium

Currently, several mite growth culture media used in the production of allergenic extracts contain animal-derived components that limit their use diagnostic and/or therapeutic applications. The aim this study was to evaluate D. pteronyssinus and farinae mites a semi-synthetic medium without proteins produce highly reproducible for purposes be more consistent with regulations health authorities. Both species showed optimal medium. highest expression allergens Der p 1 f observed at last phases growth. Semi-synthetic facilitated excellent rates house dust cultures. Adjusting cultivation time decide point processing is decisive.